|
KMID : 0356619930080010059
|
|
Journal of Korean Society of Endocrinology
1993 Volume.8 No. 1 p.59 ~ p.65
|
|
|
Effect of Dexamethasone on Proliferation, Collagen Synthesis and Alkaline Phosphatase Activity of Normal Human Bone Marrow Stromal Cells
|
|
|
|
|
Abstract
|
|
|
ABSTRACT
@EN The effect of dexamethasone on proliferation, collagen synthesis and alkaline phoshatase acitivity of cultured human bone marrow stromal cells (hBMSC) were investigated. Treatment of dexamethasone for 24h at the concentration of 10E-8M
significantly suppressed [3H] thymidine incorporation and further inhibition was observed with longer treatment (18 days) or higher concentration (10E-7M), suggesting that dexamethasone affects proliferation of hBMSC in a dose-dependent manner.
Dexamethasone (10E-8M) treatment for 48h decreased the incorporation of [3H] proline into CDP (43.7±7.9% of control) and NCP (65.2±8.4% of control), with a greater effect on CDP. Therefore % collagen synthesis was also decreased to 70.2±3.9%
of
control. Alkaline phosphatase activity of hBMSC was very high, 630±167 nmoles/mg protein/min after 4 days culture, and 1112±312 nmoles/mg protein/min after 7 days culture, which was markedly stimulated with addition of dexamethasone (10E-8M) to
191.3±21.9% and 316.9±45.7% of control, respectively.
We conclude that glucocorticoids may have a physiological role in regulating bone growth by controlling the proliferation and differentiation of osteoblast precursors. Glucocorticoid induced osteoporosis may be simply an extension of this
physiologic
suppressive effect (J Kor Endocrinol 59~65, 1993).
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|